THE INFLUENCE OF GRAVITY AND MECHANICAL FORCES AT THE CELLULAR LEVEL

Principal Investigators: Dr. Nancy D. Searby and Dr. Eduardo Almeida, NASA Ames

 

Humans are made up of many cells that perform a wide variety of functions while being subjected to the mechanical loads of daily living. For example, daily activities such as walking and running subject bone-forming osteoblasts to cyclic tension and compression via the cell-to-bone attachment points called focal adhesions. Bone continually remodels and adapts to these mechanical loads. In addition, bone-forming osteoblasts are sensitive to changes in gravity, from microgravity to hypergravity; this sensitivity may be the key reason astronauts lose bone.  The objective of our research is to examine the cellular response to these changes in mechanical loading.  Cells may respond by activation of key structural and signaling elements within the cell.  Likely candidates for mediating cellular responses to mechanical loads include the cellıs skeleton, or cytoskeleton, cell surface adhesion receptors, and a complex array of intracellular signaling molecules regulated by tyrosine phosphorylation.

 

The Research Associate will participate in a project in which mechanical loads are altered and the cellular response studied.  The project may involve development of experiment unique hardware necessary to complete the investigation, such as customized cell chambers or automated devices for use on the centrifuge.  The Research Associate will assist in hardware development, perform cell biology experiments, and analyze data.  The specifics of the project will depend on the Research Associateıs background.  Background in cell biology and/or engineering, and general laboratory experience is desirable.

 

 

SIMULATION TESTBED FOR ROBOTIC EXPLORATION OF A MARTIAN SURFACE

Principal Investigators: Dr. Jeffrey Smith, Dr. Richard Boyle, Dr. Xander Twombley

 

This Research Associate(s) will develop a computer simulation testbed for robotic exploration of the Martian surface. With this physically-based software simulation, the student(s) will test a variety of design options for future Mars rovers, including walking and wheeled locomotion systems to search for potential evidence of life. The overall goal is to develop a realistic simulation of biological exploration using autonomous tools at remote sites. To accomplish this goal requires a diverse team of theorists, engineers and biologists. This mission supports the Astrobiology goal to search for life, or the building blocks of life, beyond Earth.

 

One or two Research Associates will be chosen for their potential contribution to the team effort, which aims to produce publishable results in a ten-week period. The team will consist of a Research Associate(s) and NASA Ames staff with an appropriate skill mix that spans three major areas: 1) knowledge of biology and the search for life and its building block on Mars, 2) engineering of Mars rover systems and 3) software development of physically-based computer simulations.  The investigators will look for a Research Associate(s) with expertise in any of the following areas:

 

·               Computer Science: develop C++ software for robotic simulation using current tools available at the BioVIS Technology Center;

·               Mechanical Engineering/Robotics: work with real robotic systems and provide input to simulation system with validated robotic data results;

·               Neuro-Engineering: develop evolutionary robotics control systems based on genetic algorithms for navigation of simulated terrain and completion of mission objectives;

·               Geology/Chemistry/Biology: develop sensor/detector requirements of a targeted mission, such as a sample return mission to Mars. Use these scientific objectives to define success criteria for simulated robotic systems.

 

Objectives of this project:

 

·               Identify current and future walking and wheeled rover designs under consideration for future robotic exploration of Mars. Focus on a future Mars Sample Return mission.

·               Identify Astrobiology mission objectives that rovers must accomplish, focusing on a future Mars Sample Return mission.

·               Modify existing Mars rover designs in simulation software to include current design considerations.

·               Develop self-guided control mechanisms with the simulation environment.

·               Identify and design specific sensors for navigational control and for data collection.

·               Explore new, original design options considered by the team.

·                Perform validation testing of appropriate design options in a Mars-terrain mock-up using design options that have existing hardware to test.

 

 

 

LINKING PATTERN TO PROCESS USING REMOTELY SENSED IMAGERY OF VEGETATION

CANOPIES

Principal Investigator: Dr. Jennifer Dungan

 

The NASA Ames Code SGE Ecosystems Science and Technology Branch uses images of the Earth's surface collected from space to research natural processes of plant life as well as human activity. A few of our current projects include observing fires across North America, making real-time measurements of agricultural productivity and modeling the seasonal greening of the biosphere. The summer research associate will assist with a project on vegetation canopies as depicted by satellite images. The project is concerned with what explains the spatial patterns seen in images of vegetation canopies and whether these patterns are unique or can be summarized using metrics that would enable the measurement of biomass amount or extent. Students with backgrounds in any Earth or environmental science such as ecology or geography would be well-suited to this work. In addition, student should have computer programming experience.

 

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CHARTING THE HISTORY OF EARTHıS EARLIEST MICROBIAL ECOSYSTEMS

Co-Investigators: David Des Marais, Dr. Leslie Prufert-Bebout, and Linda Jahnke, NASA Ames Research Center

 

Microorganisms are the primary engines of our biosphere, and so they play major roles in the biogeochemical cycles of carbon, oxygen, nitrogen, sulfur and metals. The hierarchical organization of microbial ecosystems determines the rates of processes that shape Earth's environment, create the sedimentary and atmospheric signatures (biosignatures) of life, and define the stage upon which major evolutionary events occurred. To learn how microbes fulfill these roles on Earth and, potentially, other worlds, we must therefore understand the structure and function of microbial ecosystems. Photosynthetic microbial mats have been major players for billions of years. They are self-sustaining, complete ecosystems in which light energy that is absorbed over part of a diel (24 hour) cycle drives the synthesis of spatially organized, diverse biomass. Thus microbial mats offer an opportunity to study how microbial populations associate to control the biogeochemical cycles.

 

This project involves experiments with cyanobacterial microbial mats that are maintained in a simulated natural environment. We will explore various conditions that represent stages in the long-term (billion-year) evolution of Earth's environment. The effects of seawater composition, oxygen and dissolved inorganic carbon contents will be measured for ecosystem properties such as population sizes, elemental cycling and gas production. We will seek a better understanding of how the environment influences biosignatures such as atmospheric gases and also chemicals and minerals preserved in sedimentary rocks.

 

The student will participate in experiments with microbial mats as part of a team. He/she will measure rates of growth and migration, as well as the production and consumption of various key chemical compounds using microelectrodes and chromatographs. These measurements will be interpreted as components of ecosystem processes that can vary in response to changing environmental conditions. The student will thereby contribute to an improved understanding of how ancient photosynthetic ecosystems interacted with changing environments and recorded their legacy.

 

#1 FLOW DYNAMICS IN MICROBIAL ECOSYSTEMS

This project will focus on looking at the dynamics of water flow on the physical integrity and structure of microbial communities (microbial mats and stromatolites).  Focus will be on the effects of physical flow dynamics on sedimentation, diffusion, microbial growth and gas flux in microbial systems.

 

#2 ENVIRONMENTAL EFFECTS ON MICROBIAL GROWTH AND BIOMARKER PRODUCTION.

This project will examine the effects of various growth conditions (irradiance, nutrients, temperature, flow conditions) on motility and biomarker production in cyanobacteria.

 

Coursework in chemistry and/or biology, and a working knowledge of word processing and spreadsheets, is necessary.

 

 

EXTRATERRESTRIAL ICE CHEMISTRY: THE ORIGIN OF LIFE AND FALSE BIOMARKERS

Principal Investigator: Dr. Max Bernstein, NASA Ames

 

The tons of organic material that come to the Earth every day on meteorites and dust particles probably helped to make the Earth habitable, and possibly played a role in the origin of life.  The organic compounds in meteorites (which are better characterized than those in dust particles) include amino acids (basic components of proteins) purine and pyrimidine bases (basic components of DNA and RNA), as well as a host of other complex organic molecules that resemble those that make up membranes and play key roles in our biochemistry.  The origin of many of these molecules remains mysterious, but in recent years we have performed experiments that suggest low temperature radiation chemistry could account for many of these chemicals.

 

We investigate the formation and distribution of organic molecules in space and consider the impact such molecules may have had on the of origin life on Earth and the search for life on other planets.  We perform laboratory experiments to ascertain what kind of chemistry can be done by radiation processing of low temperature ices. These experiments allow us to assess whether extraterrestrial ices of the kind seen in interstellar space and the Solar System (such as on Europa and comets) are the birthplace of organic molecules such as and amino acids (Nature, 2002, 416, 401­403), amphiphiles (Astrobiology, 2003, 2, 371, Proc. Nat. Acad. Sci. 2001, 98, 815), quinones (Science, 1999, 283, 1135) and other functionalized aromatic compounds (Meteoritics, 2001, 36, 351; ApJ., 2003, 582, L25; ApJ., 2002, 576, 1115).

 

Understanding how components of proteins and DNA could form in sterile space environments is also of relevance to our search for life elsewhere in the Solar System, the great task now ahead for NASA. If we find evidence of Life elsewhere in the Solar System it will probably be in form of chemical biomarkers, quintessentially biological molecules that indicate the presence of micro-organisms. While most people think of molecules, such as amino acids, and nucleo-bases, as good candidate biomarkers, these molecules are produced non-biotically in space and are expected to be present on the surface of other planets, even in the absence of Life. Understanding the range of non-biological organic molecules that could act as false biomarkers in space is a prerequisite for any reasonable search for biomarkers on other worlds.

 

Highly qualified candidates should combine laboratory experience with a knowledge of, or an interest in, space science applications that relate to astrobiology.

 

 

ADAPTING TO UNUSUAL SENSORY ENVIRONMENTS

Principal Investigator: Dr. Robert B. Welch, NASA Ames

 

NASA is committed to the use of virtual reality and teleoperator systems (e.g., the Space Stationıs robotic arm) for training, simulation, and other tasks necessary in space.  Unfortunately, however, many of these interactive technologies suffer from defects causing users to experience initial misperceptions and errors in hand-eye coordination.  These technological flaws and their perceptual and behavioral consequences will be with us for many years to come and some may never be resolved.

 

The present research examines the ability of humans to adapt to an analogue of a virtual environment or teleoperator system in which a transformation between hand movements and visual feedback exists.  A real life example is when an astronautıs rightward movement of a joystick causes the robotic arm to move off in a very different direction.  A laboratory analogue is one in which in-out movements of a computer mouse cause a cursor viewed on a monitor to move off at a 40-degree angle, rather than its normal up-down path.  Another entails the use of a prism to displace the visual field to one side, thus causing initial errors when attempting to reach for targets.  We will investigate (1) the course of visual-motor adaptation to unusual sensory environments such as these, (2) the extent to which this adaptation is facilitated or inhibited by various factors known to influence other types of adaptation, and (3) the distinction between adaptation based on perceptual recalibration and adaptation based on visual-motor skill acquisition. 

 

 

 

 

BIOGEOCHEMISTRY OF MICROBIAL ECOSYSTEMS

Principal Investigator: Dr. Tori Hoehler, NASA Ames

 

For more than 75% of its history, Earthıs biosphere consisted exclusively of microorganisms.  During all of this time, microorganisms represented the sole biological agents of chemical change on Earthıs surface, and they continue to play a preeminent role in global chemical cycles into the modern day.  If Earthıs history is any guide, our searches for fossil or extant life on other worlds are more likely to encounter evidence of microbial life than of ³higher² organisms.  The farther from home we look for life, the more our search will depend on exclusively chemical forms of evidence. With this context, our group seeks to better understand the way in which microbial life affects the chemistry of its host planet.  The systems we study must, of necessity, be terrestrial and modern.  Wherever possible, however, we focus on systems that provide useful analogs of Earthıs microbially-dominated past, and seek to guide our studies and place our results in the context of astrobiological search strategies.

 

The summer researcher will aid in examining the factors controlling production and flux of potential biosignature gases from one or more systems including:  hypersaline microbial mats (as analogs of the vast ³reefs² of cyanobacteria that dominated biological productivity on Earth for as much as two billion years); anoxic sediments (as the ultimate biological buffer on Earthıs oxidation state, and the ultimate filter on material passing into the rock record); chemosynthetic, anaerobic communities (as potential analogs of Earthıs earliest, pre-photosynthetic life forms).  Daily activities will consist largely of analytical characterization of gas fluxes from these communities, using a variety of modern analytical tools.  Significant time will also be spent discussing the general scientific and astrobiological context for the specific systems under study.  Because much of our focus is on understanding the thermodynamic controls on biogeochemistry, a strong background in chemistry, including lab work, would be beneficial for a prospective summer researcher in our group.

 

 

 

TWO PROJECTS IN BIOPHYSICS

Principal Investigator: Dr. Wenonah Vercoutere, NASA Ames

 

#1 CELLULAR BIOPHYSICS:

In this first project, the goal is to elucidate how the structure of DNA influences its molecular dynamics and how this may affect genomic responses to the mechanical stimuli that occur because of gravity.  The hypothesis is that gene expression in mechanosensitive adherent cells is in part regulated directly by mechanical force transduction from the extracellular matrix to the nuclear matrix and protein-packaged DNA.  This work will improve the context in which we understand the ability of adherent cells to detect, filter and respond to the mechanical stimulation of gravity.

 

#2 SINGLE MOLECULE BIOPHYSICS:

In this second project, the objective is to elucidate how the dynamics of DNA structure are altered by damage caused by radiation or oxidation at the single molecule level, and determine ways to ameliorate this damage.  The chemical changes to DNA caused by ultraviolet radiation are expected to alter the molecular structure and/or dynamics sufficiently to be individually identifiable using an aqueous ion-conducting nanopore detector.  This hypothesis is based on previous work has shown that single nucleotide differences can be discriminated using such a nanopore detector.  This technique will vastly improve the ability to assess damage to DNA, and provide a simple means to help characterize the risks of radiation exposure in space.  It may also provide a method to test radiation protection.

 

 

GROUND BASED DETECTION OF TRANSITS OF EXTRASOLAR PLANETS

Principal Investigator: Dr. Tim Castellano, NASA Ames

 

Small telescopes and commercially available charge coupled device cameras are capable of achieving the necessary photometric precision to detect transits of short period Jovian sized extrasolar planets. Only 1 of the 100 or so extrasolar planets currently known has been extensively studied during its transits. Tim Castellano of NASA Ames and collaborator Greg Laughlin of The University of California at Santa Cruz have developed a collaboration with amateur astronomers, students and teachers, in order to continuously measure the brightness of many stars that harbor short period extrasolar planets. A small robotic observatory was developed last summer with the help of undergraduate physics students from the NSF REU and NAFEO programs.

 

Summer 2005 work will concentrate on refining the observatory in order to improve its ability to measure small changes in the brightness of stars indicative or an orbiting planet, surveying promising candidates, and communicating techniques and procedures to amateur astronomers, teachers and students nationwide. A website that facilitates remote collaboration with amateurs, teachers and students worldwide: www.transitsearch.org.

 

Students with a familiarity with telescopes, Astronomy and Physics coursework, computer skills (including scientific programming, web development or networking), good problem solving & laboratory skills, and a willingness to work nights(!) are encouraged to apply.

 

 

 

 

LARGE SCALE COMPUTATIONAL SCIENCE AND ENGINEERING ON THE COLUMBIA SUPERCOMPUTER

Principle Investigator: Dr. Walter F. Brooks, NASA Ames

 

NASA has recently developed and put into production a 61.0TeraFlop peak  Supercomputer, Columbia that is rated as the worlds 2nd fastest supercomputer.   The system has over 600 users and  is being used to address NASA's most challenging problems including major efforts in areas such as  Shuttle return to flight, Space science, Nanotechnology, Aeronautics, advanced Aerospace vehicles design and  Earth Science.   The system is comprised of twenty 512 processor SGI Altix  servers coupled by an infiniband fabric and supported with 440Tbytes of RAID.  A portion of the system has been coupled together to form a 2048 processor Shared Memory Processor(SMP) , the Columbia system utilizes  a Linux operating system.  Research opportunities exist in the areas of system optimizations, tool development, code porting and scaling as well as visualization.  The system will be coupled to the National Lambda Rail and additional opportunities exist in the areas of WAN and LAN development and deployment.

 

 

 

EarBot:  BIOLOGICALLY INSPIRED AUTONOMOUS ROBOTIC CONTROL SL BioVIS TECHNOLOGY CENTER

Principal Investigators: Dr. Jeffrey Smith, Dr. Richard Boyle, Dr. Xander Twombly

 

Summary

         Planetary exploration and construction will have limited on-site human resources, and must rely on the use of supervised autonomous robots to provide the capabilities required to establish a human presence.  Autonomous operation of robotic devices requires the capability to move rapidly and safely through complex environments, maintaining stability while performing tasks and acquiring scientific data.  Next generation autonomous explorers will gather information while in motion, and robots performing ground-based construction must have the capabilities to safely interact with targets and one another.

         The vestibular system is phylogenetically ancient, and enables creatures to maintain equilibrium and spatial orientation while moving freely within the environment on land, air and water.  We propose to develop the EarBot - a neural control paradigm inspired by vestibular mechanisms ­ and apply it to the control of autonomous robots.  Using the nervous systemıs unique ability to predict the effect of intended motion on its own motion sensors, the EarBot will execute compensatory stabilization commands with effectively zero execution lag time ­ a highly robust control architecture. Robots imbued with the EarBot will have greater capabilities to track moving targets (coupled with video), adjust to rapidly changing terrain (balance reflex), and stabilize instrumentation.

         The EarBot will use spatial arrays of tunable analog Micro-Electro-Mechanical Systems (MEMS) sensors as inputs to circuits that mimic the feedforward, feedback, and predictive mechanisms found in the brain, and use Field Programmable Gate Arrays (FPGAs) to implement these neural circuits.  FPGAs are reconfigurable computing, providing robust and redundant computing resources.  A major characteristic of the EarBot is an energy conservation approach that allows for adjustable, on demand power consumption. This approach is based on predictive neural mechanisms that distinguish self-generated movements from quiescence, and will extend the operational capacity for long duration missions.

 

Project Milestones

Phase I

Simulate MEMS multi-axis inertial measurement systems as inputs to a neural network model of the compensatory Vestibulo-Ocular Reflex (VOR).  Use our previously developed robot and terrain simulation environment to implement control mechanisms for stabilizing a camera mounted on a walking robotic explorer on uneven terrain, including compensation for self-directed motion.  Demonstrate efficacy of neural control concept and develop implementation plan for sensor and processing architecture.

 

Phase II

1) Construct sensor systems with low power MEMS, design triggers that transition the EarBot between high and low rates of sensor data collection, and construct neural circuitry to calculate optimal inertia measurements from sensors. Implement control system from VOR simulation in Phase I using the MEMS inputs and apply to stabilize a multi-axis camera system.  FPGA implementations of neural circuitry.

 

2)  Determine optimal geometry of sensors for adaptive use of power resources during active exploration and quiescent states.  Develop predictive motion component to pre-compensate for planned motion.  Simulate balance reflex mechanism with multi-legged robot in simulation environment. 

 

3) Preflight field-testing phase - Test EarBot camera stabilization on multi-legged robot and balance reflex control based on the earlier results.  Embed and test on other appropriate robotic systems.

 

Available Projects for Astrobiology Academy Participants

 

1)    Testing and characterization of MEMS sensor systems.  A multi-axis sensor system to measure translational and rotational accelerations has been constructed.  The system consists of two ³sensor heads², each comprising 6 translational degrees of freedom (redundancy on all axes) and 3 rate gyros, each of which sends signals to a TinyARM processor.  The response of these sensors systems needs to be determined and calibrated against the models used to calculate absolute position and orientation from the sensor signals, and the processor system must be programmed to process and communicate with a host system efficiently in preparation for deployment on a mobile platform.

2)    Signal processing models of sensor data.  A variety of signal processing methods are available to transform the raw sensor data obtained from the MEMS devices into absolute position and orientation data.  An investigation of the efficiency of different filtering techniques (linear adaptive, Kalman filtering, etc) must be performed to determine the required processing methods that must be implemented at both the peripheral (tinyARM) level and central (host processor) to maximize signal accuracy while minimizing processor power usage.  Signal processing models of the sensors will need to be developed in MATLAB and Simulink, and then translated to the tinyARM and host processor systems as time allows.

 

 

 

 

SPITZER SPACE TELESCOPE:  CHARTING THE STARDUST PATH TO OUR FUTURE

Principle Investigator:  Yvonne Pendleton, NASA Ames Research Center

 

The overall goal of this project is to develop high quality, easy to use web-based materials which include new activities and descriptions of a multitude of Spitzer Space Telescope images that can be integrated into the Voyages Through Time (VTT) lessons which are already available through the following web site: http://www.voyagesthroughtime.org.

 

Our intent is to create useful, highly relevant and timely educational materials from Spitzer results, and to cast a wide net to reach teachers and students quickly.  Two Academy students will work with scientists and educators to develop ideas for activities and lessons based on Spitzer data.  The material already developed for VTT during the four year long development process will serve as an excellent jump start, illustrating both the level and content their lessons should contain.  The lessons created by the Academy students will be reviewed by the evaluation services provided through the Origins EPO Forum at Space Telescope Science Institute.

 

This project will bring together the strength of the NASA Academy at Ames Program, the highly successful integrated science curriculum Voyages Through Time (SETI Institute), and the astonishing results of the Spitzer Space Telescope in a unique manner of education outreach.  By assigning two Academy students the opportunity to update VTT materials and create new activities and web material for teachers using Spitzer results, we anticipate a rewarding and educational experience for the Academy students.  This work will also result in the development of a lasting resource for use by teachers and students worldwide which will explain and connect the Spitzer infrared world to the visible world around us.

 

 

 

NEW ABLATIVE MATERIALS FOR THERMAL PROTECTION SYSTEMS (TPS) APPLICATIONS

 

Principle Investigators:  Dr. Sylvia Johnson and Dr. Mairead Stackpoole

 

The Thermal Protection Materials and Systems (TPS) Branch is actively working toward developing new and improved TPS concepts. One of the current focuses is on development of new ablative materials for entry vehicles. Development of ablative materials has been at a very low level since Apollo and we are currently trying to address this issue. This project will focus on development of alternative lightweight ablator concepts using carbon substrates. Initial samples will be processed and key properties determined.

 

Students with a background in materials science, ceramic engineering or chemistry are encouraged to apply. Some experience in materials processing and characterization (thermal and mechanical properties) is desirable.